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bigdye ® terminator v3.1 cycle sequencing chemistry  (Thermo Fisher)


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    Thermo Fisher bigdye ® terminator v3.1 cycle sequencing chemistry
    Bigdye ® Terminator V3.1 Cycle Sequencing Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bigdye+terminator+sequencing+chemistry/bigdye+terminator+v3+1+cycle+sequencing+kit/pmc11856360-133-7-14
    Average 90 stars, based on 1 article reviews
    bigdye ® terminator v3.1 cycle sequencing chemistry - by Bioz Stars, 2026-09
    90/100 stars

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    Purification:

    Article Title: Bat Distribution Size or Shape as Determinant of Viral Richness in African Bats
    Article Snippet: .. Purified PCR products were sequenced commercially (Macrogen, Seoul, Korea) with the respective forward primer using BigDye Terminator sequencing chemistry (Applied Biosystems, Foster City, CA, USA) on ABI 3730xl sequencer. .. Sequences were edited in Sequencher 4.6 (Gene Codes, Ann Arbor, MI, USA), manually checked for correct base reading and protein coding frame, and aligned by eye in BioEdit 7.0 .

    Article Title: Mitonuclear and phenotypic discordance in an Atlantic Forest frog hybrid zone.
    Article Snippet: Amplification conditions included an initial denaturation at 94°C for 5 min, followed by 35–40 cycles of denaturation at 94°C for 1 min, annealing at 53°C for 1 min, extension at 72°C for 1 min, followed by a final extension at 72°C for 5–10 min. We purified final PCR products using 1X SAP buffer, SAP enzyme (1 unit; Life Technologies), and exonuclease I (10 units; Life Technologies). .. We sequenced purified PCR products in both directions using BigDye terminator sequencing chemistry (Applied Biosystems) on an ABI automated 3730xl Genetic Analyzer (Applied Biosystems), and obtained good quality mitochondrial sequences for a total of 302 individuals (Table 1). ..

    Article Title: Mitonuclear and phenotypic discordance in an Atlantic Forest frog hybrid zone
    Article Snippet: Amplification conditions included an initial denaturation at 94°C for 5 min, followed by 35–40 cycles of denaturation at 94°C for 1 min, annealing at 53°C for 1 min, extension at 72°C for 1 min, followed by a final extension at 72°C for 5–10 min. We purified final PCR products using 1X SAP buffer, SAP enzyme (1 unit; Life Technologies), and exonuclease I (10 units; Life Technologies). .. We sequenced purified PCR products in both directions using BigDye terminator sequencing chemistry (Applied Biosystems) on an ABI automated 3730xl Genetic Analyzer (Applied Biosystems), and obtained good quality mitochondrial sequences for a total of 302 individuals (Table ). ..

    Polymerase Chain Reaction:

    Article Title: Bat Distribution Size or Shape as Determinant of Viral Richness in African Bats
    Article Snippet: .. Purified PCR products were sequenced commercially (Macrogen, Seoul, Korea) with the respective forward primer using BigDye Terminator sequencing chemistry (Applied Biosystems, Foster City, CA, USA) on ABI 3730xl sequencer. .. Sequences were edited in Sequencher 4.6 (Gene Codes, Ann Arbor, MI, USA), manually checked for correct base reading and protein coding frame, and aligned by eye in BioEdit 7.0 .

    Article Title: MALDI-TOF mass spectrometry misidentification of Cutibacterium namnetense and Cutibacterium modestum: Implications for multiplex PCR phylotyping of Cutibacterium acnes.
    Article Snippet: Amplified products were purified using a QIAquick® PCR purification kit (QIAGEN, UK) according to the manufacturer’s instructions. .. Sequencing reactions were performed using BigDye Terminator sequencing chemistry (ThermoFisher Scientific) with the relevant forward and reverse PCR primers. .. Samples were then run on an ABI Prism® 3100 capillary electrophoresis system (ThermoFisher Scientific).

    Article Title: Mitonuclear and phenotypic discordance in an Atlantic Forest frog hybrid zone.
    Article Snippet: Amplification conditions included an initial denaturation at 94°C for 5 min, followed by 35–40 cycles of denaturation at 94°C for 1 min, annealing at 53°C for 1 min, extension at 72°C for 1 min, followed by a final extension at 72°C for 5–10 min. We purified final PCR products using 1X SAP buffer, SAP enzyme (1 unit; Life Technologies), and exonuclease I (10 units; Life Technologies). .. We sequenced purified PCR products in both directions using BigDye terminator sequencing chemistry (Applied Biosystems) on an ABI automated 3730xl Genetic Analyzer (Applied Biosystems), and obtained good quality mitochondrial sequences for a total of 302 individuals (Table 1). ..

    Article Title: Novel Compound Heterozygous Mutations in the SYNE1 Gene in a Taiwanese Family: A Case Report and Literature Review.
    Article Snippet: .. The PCR products were sequenced using Applied Biosystems BigDye Terminator sequencing chemistry and then run on an ABI3730xl genetic analyzer according to the manufacturer’s instructions (Applied Biosystems, Foster City, CA, USA). .. Sequence analysis was performed using Lasergene (DNASTAR, Madison, WI, USA).

    Article Title: Mitonuclear and phenotypic discordance in an Atlantic Forest frog hybrid zone
    Article Snippet: Amplification conditions included an initial denaturation at 94°C for 5 min, followed by 35–40 cycles of denaturation at 94°C for 1 min, annealing at 53°C for 1 min, extension at 72°C for 1 min, followed by a final extension at 72°C for 5–10 min. We purified final PCR products using 1X SAP buffer, SAP enzyme (1 unit; Life Technologies), and exonuclease I (10 units; Life Technologies). .. We sequenced purified PCR products in both directions using BigDye terminator sequencing chemistry (Applied Biosystems) on an ABI automated 3730xl Genetic Analyzer (Applied Biosystems), and obtained good quality mitochondrial sequences for a total of 302 individuals (Table ). ..

    Article Title: A Novel Homozygous Loss-of-Function Variant in SPRED2 Causes Autosomal Recessive Noonan-like Syndrome
    Article Snippet: .. PCR products were double-strand sequenced using BigDye Terminator sequencing chemistry (Life Technologies, Carlsbad, CA, USA) and analyzed on an ABI 3130xL automatic DNA sequencer (Life Technologies). ..

    Sequencing:

    Article Title: Bat Distribution Size or Shape as Determinant of Viral Richness in African Bats
    Article Snippet: .. Purified PCR products were sequenced commercially (Macrogen, Seoul, Korea) with the respective forward primer using BigDye Terminator sequencing chemistry (Applied Biosystems, Foster City, CA, USA) on ABI 3730xl sequencer. .. Sequences were edited in Sequencher 4.6 (Gene Codes, Ann Arbor, MI, USA), manually checked for correct base reading and protein coding frame, and aligned by eye in BioEdit 7.0 .

    Article Title: Species delineation using Bayesian model-based assignment tests: a case study using Chinese toad-headed agamas (genus Phrynocephalus )
    Article Snippet: .. All DNA sequencing reactions were performed using BigDye terminator sequencing chemistry with an ABI 3730 sequencing machine (Applied Biosystems). ..

    Article Title: MALDI-TOF mass spectrometry misidentification of Cutibacterium namnetense and Cutibacterium modestum: Implications for multiplex PCR phylotyping of Cutibacterium acnes.
    Article Snippet: Amplified products were purified using a QIAquick® PCR purification kit (QIAGEN, UK) according to the manufacturer’s instructions. .. Sequencing reactions were performed using BigDye Terminator sequencing chemistry (ThermoFisher Scientific) with the relevant forward and reverse PCR primers. .. Samples were then run on an ABI Prism® 3100 capillary electrophoresis system (ThermoFisher Scientific).

    Article Title: Mitonuclear and phenotypic discordance in an Atlantic Forest frog hybrid zone.
    Article Snippet: Amplification conditions included an initial denaturation at 94°C for 5 min, followed by 35–40 cycles of denaturation at 94°C for 1 min, annealing at 53°C for 1 min, extension at 72°C for 1 min, followed by a final extension at 72°C for 5–10 min. We purified final PCR products using 1X SAP buffer, SAP enzyme (1 unit; Life Technologies), and exonuclease I (10 units; Life Technologies). .. We sequenced purified PCR products in both directions using BigDye terminator sequencing chemistry (Applied Biosystems) on an ABI automated 3730xl Genetic Analyzer (Applied Biosystems), and obtained good quality mitochondrial sequences for a total of 302 individuals (Table 1). ..

    Article Title: Novel Compound Heterozygous Mutations in the SYNE1 Gene in a Taiwanese Family: A Case Report and Literature Review.
    Article Snippet: .. The PCR products were sequenced using Applied Biosystems BigDye Terminator sequencing chemistry and then run on an ABI3730xl genetic analyzer according to the manufacturer’s instructions (Applied Biosystems, Foster City, CA, USA). .. Sequence analysis was performed using Lasergene (DNASTAR, Madison, WI, USA).

    Article Title: Mitonuclear and phenotypic discordance in an Atlantic Forest frog hybrid zone
    Article Snippet: Amplification conditions included an initial denaturation at 94°C for 5 min, followed by 35–40 cycles of denaturation at 94°C for 1 min, annealing at 53°C for 1 min, extension at 72°C for 1 min, followed by a final extension at 72°C for 5–10 min. We purified final PCR products using 1X SAP buffer, SAP enzyme (1 unit; Life Technologies), and exonuclease I (10 units; Life Technologies). .. We sequenced purified PCR products in both directions using BigDye terminator sequencing chemistry (Applied Biosystems) on an ABI automated 3730xl Genetic Analyzer (Applied Biosystems), and obtained good quality mitochondrial sequences for a total of 302 individuals (Table ). ..

    Article Title: A Novel Homozygous Loss-of-Function Variant in SPRED2 Causes Autosomal Recessive Noonan-like Syndrome
    Article Snippet: .. PCR products were double-strand sequenced using BigDye Terminator sequencing chemistry (Life Technologies, Carlsbad, CA, USA) and analyzed on an ABI 3130xL automatic DNA sequencer (Life Technologies). ..

    DNA Sequencing:

    Article Title: Species delineation using Bayesian model-based assignment tests: a case study using Chinese toad-headed agamas (genus Phrynocephalus )
    Article Snippet: .. All DNA sequencing reactions were performed using BigDye terminator sequencing chemistry with an ABI 3730 sequencing machine (Applied Biosystems). ..



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